Phenotypic screening by High Content Screening monitors all modification of phenotypic changes. The interest of this technology is having a significant content of biological information (phenotypic and target modifications) and this, cell by cell. This important content of information results in a very high amount of data that must be analyzed globally (with PCA reduction for example). Thus, the data analysis and statistics, especially the Z-score that everybody knows, need to be reviewed. Guyon et al boast Φ-score. What do you think about that?
“Using robust statistics and a variance model, we demonstrated that the Φ-score showed better sensitivity, selectivity and reproducibility compared to classical approaches. The improved performance of the Φ-score paves the way for cell-based screening of primary cells, which are often difficult to obtain from patients in sufficient numbers.”