We just begin a test period of the Operetta “High Content Imaging System” from Perkin Elmer. First impressions are very good, especially using confocal option for 3D imaging. As you can see below, neurites growth is quite easy to observe around and inside a spheroid of SH-SY5Y cells. To properly count it is more complicated for the moment but we progress in the mastering of “Harmony” and “Columbus” softwares, as for “Acapella” scripts. Our next challenge is to build comprehensive 3D visualisation with Volocity software.
Hoechst + Beta III tubulin
Sliding Parabola filter on Beta III tubulin channel
Ridge filter on Beta III tubulin channel
Reconstructed picture usable for neurites count
Reconstructed picture usable for neurites count
Sliding Parabola filter on Beta III tubulin channel
Ridge filter on on Beta III tubulin channel
Hoechst + Beta III tubulin
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