The creation of a double strand break (DSB) is accompanied by the phosphorylation of histone H2AX. The measurement of serine 139 phosphorylated histone H2AX (γH2AX) is reported to be a marker of interest to identify potential genotoxic activity.

In order to evaluate the High Content Screening for γH2AX detection, 4 non genotoxic compounds and 9 genotoxic compounds from the ECVAM list I or II were selected to to be tested on HepG2 cell line. These cells offer the advantage to have H2AX expression data in the literature. In parallel, Human primary keratinocytes were included. Indeed these cells would be relevant for investigating skin adverse effects of topical applied xenobiotics with the advantage of High content imaging as valuable tool for screening in the early discovery phase.

View and download on Slideshare (low quality) :
http://fr.slideshare.net/hcspharma/poster-development-of-double-stand-break-assessment-assay-with-hcs-by-using-the-hepg2-cell-line-and-evaluation-with-primary-skin-cell-nhek

Ask for high quality link by putting your email below


1 Comment

Genotoxicity assay development in collaboration with Galderma R&D | HCS-pharma · March 16, 2017 at 10:07 am

[…] Rennes, We had the opportunity to present our work on genotoxicity assay (comet assay on TK6 & gH2AX on keratinocytes and HepG2) in collaboration with the team of in vitro of Galderma R&D. It is really exciting to work with […]

Leave a Reply