Skin microbiota is composed of several type of micro-organismes (bacteria, viruses, fungi). Most of them are “commensal” and maintain a symbiotic interaction with our skin. The balance between the different bacterial populations is important to reduce pathogenic infection. Staphyloccus epidermis is mostly present on human skin. This strain is known to produce  inhibition zones to repel a colony of overgrown bacterial causing acnee Propionibacterium acnes. Our assay allows to assess the effects of your compounds on S. epidermidis attachment on human keratinocytes.

Nuclei in blue, S. epidermidis in green

Pre exposition to compounds can be used to assess preventive effects. Exposition to compounds after S. epidermidis colonisation can be performed to evaluate your compounds innocuity on skin microbiota. You can also co-exposed your compounds to study acute protection effects. Using cell image analysis, we can assess several parameters including :

  • Live/Dead
  • Cells inflammation
  • Oxidative stress
  • Pathogen infection
  • And more !