NFkB (Nuclear Factor kappa-light-chain-enhancer of activated B cells) is a key player in the inflammatory response. It belongs to the category of “rapid-acting” primary transcription factors since it is present in cells in an inactivated state, is quickly activated and is a first responder to pro-inflammatory stimuli. Using an inflammatory inducer, we can assess the anti-inflammatory of your compounds.
Pre-exposition to compounds can be used to assess preventive effects. Exposition to compounds after LPS stimulation can be performed to evaluate acute protection effects.
You can multiplex this assay by monitoring pro- and anti-inflammatory pathways with interleukin secretion !
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