Generation of Reactive Oxygen Species (ROS) occurs at a controlled rate in healthy cells, but can be dramatically increased under oxidative stress. ROS are responsible for many deleterious effects, like alteration of proteins, nucleic acids and membrane lipids. Chronic oxidative stress is associated to pathologies like neurodegenerative diseases, carcinogenesis or atherosclerosis, and more generally to ageing. ROS can be measured directly in situ in cells by using stains that fluoresce only when oxidized.
Pre-exposition to compounds is used to assess preventive effects. You can also co exposed your compounds to study acute protection effects. Using cell image analysis, we calculated anti-oxidative effects of your compounds compare to N-acetyl-cystein.
If you are interested by studying the anti-oxidative properties of your compounds, don’t hesitate to contact us for a personal quotation !