Why choosing BIOMIMESYS® 3D technology for in vitro assays ?
It is well established that the extra-cellular matrix (ECM) plays a central role in cell homeostasis and cell adhesion by providing support, cell-to-cell communication and differenciation (or dedifferenciation and tumoral progression when ECM is modified). The stiffness and elasticity of the ECM has important implications in cell migration, gene expression and differentiation. Component of the ECM are produced intracellularly by resident cells and secreted into the ECM via exocytosis. The ECM includes the interstitial matrix and the basement membrane. ECM is formed by gels of polysaccharides and fibrous proteins.
Hyaluronic Acid (HA) is an essential component of the ECM. HA is a polysaccharide consisting of alternating residues of D-glucuronic acid and N-acetylglucosamine, and unlike other GAGs, is not found as a proteoglycan. HA in the extracellular space confers upon tissues the ability to resist compression by providing a counteracting turgor force by absorbing significant amount of water. Hyaluronic acid is thus found in abundance in the ECM of load-bearing joints. It is also a chief component of the interstitial gel.
HA acts as an environmental cue that regulates cell behavior during embryonic development, healing processes, inflammation, and tumor development. It interacts with a specific transmembrane receptor, CD44.
BIOMIMESYS® 3D technology (patent)
BIOMIMESYS® 3D technology is a natural Hyaluronic Acid-based scaffold to better mimic the tissue microenvironment and provide more predictive tools for drug development. The BIOMIMESYS® products are formed by crosslinking reaction of hydrosoluble modified hyaluronic acid and others extracellular matrix components (ECM) with ADH (Adipic acid dihydrazide) to form the extra-cellular matrix. Depending on the organ, extracellular matrix components are different and at various rates allowing a more or less dense and compact cellular environment. BIOMIMESYS® 3D technology allows us to reproduce each cell environment of each organ.
BIOMIMESYS® Adipocytes 3D technology is a reproduction of ECM from adipose tissue consisting of Hyaluronic Acid-based scaffold including RGDS, Collagen I and collagen VI.
In this context of 3D culture, preadipocyte cells differenciate in adipocytes with accumulation of triglycerides.
To learn more, read this article : Louis et al (2017) A biomimetic hydrogel functionalized with adipose ECM components as a microenvironment for the 3D culture of human and murine adipocytes. Biotechnol Bioeng. 2017 Aug;114(8):1813-1824.
BIOMIMESYS® Hepatocytes 3D technology is a reproduction of ECM from liver tissue consisting of Hyaluronic Acid-based scaffold including RGDS,Galactose, Collagen I and collagen IV.
In this context of 3D culture, primary human hepatocytes maintain their differentiation and thus their activity for at least 1 month.
Mimicking Natural ECM, BIOMIMESYS is a smart technology to evaluate your molecules on 3D cellular in vitro models.
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