Why choosing BIOMIMESYS® 3D technology for in vitro assays ?

Context

It is well established that the extracellular matrix (ECM) plays a central role in cell homeostasis and cell adhesion by providing support, promoting cell-to-cell communication and differentiation (or dedifferentiation and tumoral progression when the ECM is modified). The composition but also the stiffness and elasticity of the ECM have important implications in cell migration, gene expression and differentiation. Components of the ECM are produced intracellularly by resident cells and secreted into the ECM via exocytosis. The ECM includes the interstitial matrix and the basement membrane. The ECM is formed by gels of polysaccharides and fibrous proteins.

Hyaluronic Acid (HA) is an essential component of the ECM. It is a polysaccharide consisting of alternating residues of D-glucuronic acid and N-acetylglucosamine, and unlike other glycosaminoglycans (GAGs), is not found as a proteoglycan. HA in the extracellular space confers to tissues the ability to resist compression by providing a counteracting turgor force and  absorbing significant amounts of water. Hyaluronic acid is thus found in abundance in the ECM of load-bearing joints for example. It is also a chief component of the interstitial gel. HA acts as an environmental cue that regulates cell behavior during embryonic development, healing processes, inflammation, and tumor development. It interacts with a transmembrane receptor, CD44.

 

BIOMIMESYS® 3D technology (patented)

BIOMIMESYS® 3D technology is a natural HA-based scaffold to better mimic the tissue microenvironment and provide more predictive tools for drug development. The BIOMIMESYS® products are formed by crosslinking reaction of hydrosoluble modified hyaluronic acid and others extracellular matrix components (ECM) with ADH (Adipic acid dihydrazide), to form the extra-cellular matrix. Depending on the organ, extracellular matrix components are different and at various rates, allowing a more or less dense and compact cellular environment. BIOMIMESYS® 3D technology allows us to reproduce the cell environment of each organ.

To mimic the ECM, we Need to know the physicochemical properties (mechanical & composition) in normal and pathological organs :

  • mechanical properties : elastic modulus of organs’ ECM

  • composition of ECM : During different pathologies (like liver fibrosis or cancer), the secretion of collagen and GAGs is modified, therefore modifying the stiffness of the environment

Ex : Modification of the ECM of the liver during the different steps of liver disease : steatosis, NASH, fibrosis, cirrhosis

Ex : Disruption of ECM during tumor progression

For each organ we can develop a specific scaffold ECM, by fine tuning its elasticic modulus and its composition.

 

BIOMIMESYS® Adipocytes

BIOMIMESYS® Adipocytes 3D technology is a reproduction of  ECM from adipose tissue consisting of HA-based scaffold including RGDS, collagen I and collagen VI.

When human white preadipocytes are growing in BIOMIMESYS® Adipocytes matrix, the cultured adipocytes cells form clusters like native adipose tissue from human abdomen.

 

In this context of 3D culture, preadipocyte cells differentiate into adipocytes, as seen by the accumulation of triglycerides.

To learn more, read this article : Louis et al (2017) A biomimetic hydrogel functionalized with adipose ECM components as a microenvironment for the 3D culture of human and murine adipocytes. Biotechnol Bioeng. 2017 Aug;114(8):1813-1824.

BIOMIMESYS® Hepatocytes

BIOMIMESYS® Hepatocytes 3D technology is a reproduction of  ECM from liver tissue consisting of a HA-based scaffold including RGDS, galactose, collagen I and collagen IV.

 

In this context of 3D culture, primary human hepatocytes maintain their differentiation and thus their activity for at least 1 month.

 

Mimicking natural ECM, BIOMIMESYS® is THE smart technology to build more relevant 3D cellular in vitro models to test your compounds !

 

If you want to know more about BIOMIMESYS®, contact us!