In this article, authors emphasize the importance of standardisation in the development of HCS. Standards (for Image File Format, Data Integrity and Reference Material) are critical to assure both data quality and reproducibility of assays.

The scientific community must embrace the urgent need for standardization from cells to HCS data to enhance reproducibility and reduce unnecessary economic loss. Together, the scientists and end users partnering with the manufacturing companies, colleagues, and government agencies can strive to build the necessary toolboxes and protocols to ensure the highest quality of all aspects of HCS data.

Source: Standardization of High Content Imaging and Informatics