Cell biology is part of the R&D activity in dermocosmetology, as providers of active extracts or pure compounds have to demonstrate their efficacy and safety. At HCS Pharma, we are developing new dermocosmetology assays on relevant cellular models, like human primary keratinocytes and human primary fibroblasts, using our automated platform and high content analysis system. Through the use of 96 or 384 wells plates combined with process automation, we constantly increase the throughput in order to allow proof of concept assays of one or several compounds up to the screening of large compounds libraries, for single endpoint or live cells analysis to multiplexed phenotypic screening.
Assessment of compounds activity/safety can be performed on several parameters in parallel with a multiplexed assay. Among them, we describe here wound healing, inflammation assay & extra cellular matrix analysis, which are routinely performed. Other parameters can be analysed on demand on live and fixed cells, using chemical probes or immunocytochemistry.

View and download on Slideshare (low quality) : https://www.slideshare.net/hcspharma/in-vitro-dermocosmetology-high-content-analysis-approach-using-human-primary-keratinocytes-and-fibroblasts

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Cell biology is part of the R&D activity in dermocosmetology. At HCS Pharma, we are developing new democosmetology assays on human primary keratinocytes using our automation plateform and high content analysis suite.

Assessment of compounds activity/safety can be performed on several parameters (wound healing, hydration, inflammation, toxicity, genotoxicity). Further parameters could be analyzed on demand using probes and immunocytochemistry.

Thought the use of high content analysis technologies, we can provide a wide range of results from phenotypes quantification cell by cell to live cells imaging movies.

View and download on Slideshare (low quality) : http://fr.slideshare.net/hcspharma/in-vitro-dermocosmetology-high-content-analysis-approach-using-human-primary-keratinocytes

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Cell biology is part of the R&D activity in dermocosmetolgy. At HCS Pharma, we are developing dermocosmetology assay using our automation platform and high content analysis software.

Wound healing is a complex process resulting in new tissue formation, and skin remodeling. We have developed an in vitro automated assay in 96 well-plate using human primary epithelial keratinocytes in order to avoid expensive used of animal models, reduce the cost of assay and increase the volume of compounds tested.

This assay, also called “The scratch assay” is relevant to evaluate the efficacy of active ingredients for irritated skins as well as for stretch marks. In dermocosmetology, this assay is commonly used to assess compounds efficiency on wound healing process.

aquaporinewound-healing

If you want to know more on this assay, follow this link to our page on the website or contact us!

 

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