Exemple of BAP : HepG2 cells were exposed 24 hours to increasing concentrations of Benzo[a]pyrene (B[A]P).

Distinguishing aneugenic (chromosomes loss) from clastogenic effects (DNA damages) is essential to assess genotoxic properties of compounds.

In order to help you taking the right decisions for your compounds, we developed a multiplex assay in order to discriminate aneugenic from clastogenic and cytotoxic effects of your compounds in one single assay !

Based of the combined assessment of micronuclei and the phosphorylation of histone H2AX and H3, our assay allows to distinguish between clastogenic and aneugic genotoxic effects after only 24 hours of exposure to your compounds.

This assay is already validated on HepG2 cells with our in-house genotoxicity library. If you want to study other cells lines, including primary or iPS cells, we also propose validation services for this multiplex assay.

By combining the outputs, we propose a fast and cheap assay for your genotoxicity studies!

Check our genotoxicity services on our website, and ask for our validation report.

Automated Comet assay

The comet assay is a sensitive, well established technique for quantifying DNA damage in eukaryotic cells. Compatible with the detection of a wide range of DNA damaging agents, its principle consists in migration of fragmented DNA in an electrophoresis gel (damaged DNA forming the tail of the comet), while intact DNA moves at a slower rate (head of the comet). The percentage of fragmented DNA in the comet tail is a direct measure of DNA damage.

Micronucleus and H2AX assay

In response to clastogen DNA double strand breaks, H2AX is phosphorylated within minutes at Ser 139, and is then referred as γH2AX. Contrary to γH2AX, micronuclei are a late marker of damages either to DNA and be related to aneugens nor clastogens effect. By detecting both H2AX and micronuclei on our high content screening plateform, our assay provide a efficient tool to assess the genotoxicity properties of your compounds.