During these last two weeks, we took control of our new robotic platform and validated the pipetting volumes and the entire process by testing it with a few plates. Some steps are still to be improved, such as the scheduling of the plates. The first screening with a few plates was on hepatotoxicity assay on SCREEN-WELL® Hepatotoxicity library from ENZO (238 compounds) in one concentration.

After improving our process, we will test this library in dose effect in addition to the Prestwick library. We will also perform neurotoxicity assay in a screening mode on internal and Prestwick library. These assays will allow us to validate all our process to cell culture until data analysis in addition to exemplify data on our toxicity assays.

Nathalie and Julian were present during ELRIGfr event last week. They presented their company, their work and their collaborations. They have also presented two posters : the first one on a co-culture model of Parkinson and first results in 3 dimensions ; the other one on genotoxicity and steatosis assays on HepG2 cells. These next two to three months will be dedicated to validate these assays. New assays  will also be undertaken, like transporters assays, cholestasis or phospholipidosis, on HepG2 cells as well as on primary hepatotocytes. Genotoxicity assays are under validation on different cell lines as HepG2, SH-SY5Y, HeLa, HT29 cells. If you want more details, don’t hesitate to contact us.

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