In Nature Methods from July, Xi Long and colleagues from the Howard Hughes Medicale Institute Janelia Research Campus (Ashburn, Virginia, USA) have developed a RNA fluorescence in situ hybridization (FISH) to study brain development and function, which require the expression of select genes at specific times in appropriate neurons.

We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).

Source : http://www.nature.com/nmeth/journal/v14/n7/full/nmeth.4309.html

Researchers from Yale university presented a new technology to increase presicion of high speed (and wide field) imagery. Using both LEDs and laser light, they minimize spacial coherence and granularity. It’s a promising way to optimize cells imaging.

La technologie développée par les chercheurs de Yale combine la luminosité des lasers traditionnels avec la qualité d’image permise par les LEDs, en ayant notamment recours à un laser à cavité chaotique. Une telle combinaison permet d’émettre une lumière puissante, mais dotée d’une faible cohérence spatiale, et de réduire ainsi considérablement la granularité.

Curated from www.atelier.net

Here is a nice example of cell imaging uses for stem cells characterisation. Different culture conditions are performed on adult stem cells and cell adhesion, proliferation, survival, and cell migration are followed by cell imaging.

Curated from www.jove.com

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