I was really happy to be invited in SIBS 2017 in Zurich last week to present our work on 3D cell culture in phenotypic screening and our use of disruptive technologies in our process (augmented reality and virtual reality). I presented our collaborative work with Dr Karim Si-Tayeb, researcher from Institut du Thorax (CHOPIN project) on differentiation of iPS in hepatocytes in 3D culture for metabolic diseases screening. Karim has also the opportunity to present a poster on CHOPIN project with more information on iPS differentiation in hepatocytes and their use for phenotypic screening.

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The presentations during this event were really of interest with demonstration of the usage of new technologies as 3D culture, microfluidics, new microscopy technologies as light sheet microscopy. All these presentations showed that the development of cellular models for screening are made more and more complex, to be more and more physiological and relevant. As an example, Blandine Avignon from Roche has presented an epithelial barrier on a chip from Mimetas including 3D matrix and microfluidics. With this new device, they should follow the integrity of the barrier in fluorescence live cell microscopy.

I want to really thanks the organizers for this invitation and for this really nice and high scientific level event. See you in 2 years in Switzerland for the next SIBS event!

As last year, we will be very happy to attend Drug Discovery 2017 event in Liverpool (Innovation Zone, IZ1 booth). We will present our activities, the new assays that we have developed recently and our brand new robotic platform.

“Drug Discovery is ELRIG’s flagship event. Now in its 11th year, we return to ACC in Liverpool to bring together the leading lights in drug discovery research across pharma, biotech, academia and the vendor community.”

As you can see on the dedicated webpage, the event program is highly interesting:

  • Advances in Imaging
  • Drug Discovery in the 4th Dimension
  • Innovations in Assay Design, Development and Screening

And of course, don’t forget the amazing “Innovation Zone”, the right place to discover future partners, as HCS Pharma !

Come and join us in Drug Discovery 2017 in the Innovation Zone at booth IZ1, to discuss with Nathalie, our CEO and Pierre-Jean, our in vitro toxicology expert !

Nathalie will be present tuesday 3 October, 3-4 pm, on booth of our partner Molecular Devices (#D18) to answer your high-content screening (HCS) questions.  Book a time slot to meet Nathalie !

Imaging inner cells in a 3D spheroïd requires a well adapted microscopy technology. HCS Pharma is now equipped with MicroXLS Confocal from Molecular Devices to perform the best images using an automated spinning disk microscope. We also pursue our development using brand new microscopy technologies to have an overview of the different available possibilities. Thanks to Dr Sibarata and Dr Galland from the Interdisciplinary Institute for Neuroscience located in Bordeaux, France, and Mr Clouvel from Imagin Optics, who gave us the opportunity to use the SPIM microscope. Dr Savary, our partner in Innovcell 3D project, joined us with hepatocyte spheroïds prepared for imagery.

Images obtained showed us a deep imaging of our spheroïds, with the greatest contrast at the level of the first cell layers. We will go on with innovative sample preparations to allow a deeper observation of the spheroids.

I enjoyed to participate to this event hold in Stevenage at GSK, in a beautiful and impressive setting. The combination of uses of new technologies as high content imaging (HCA/HCS), imaging mass-spectrometry or imaging flow cytometry and new data analysis as machine learning and deep learning were presented in this event. These combinations show an extremely nice perspective in pharmaceutical research and in personalized medecine. I was really impressed by the quality of the presentations. James Pilling (Aztrazeneca) showed cardiotoxicity assay on 3D spheroid by using HCS technology. Emmanuel Gustin (Janssen) presented phenotypic screening by using phingerprints and machine learning. Rod Benson (Imagen Therapeutics) talked about precise and personnalised medecine for cancer by using HCS technologies.

All these emerging technologies and data analysis systems promise interesting discoveries in the near future.

I was present at the last congress ELRIGfr in Brussels. It was a strong exchange place with interesting presentations around robotics. It is always really interesting to see examples of innovative automation which give really robust results. For example, Frank Gudermann from university of Bielefield in Germany showed that automation of culture cell with cell count by holographic picture gives more robust results as manual count by trypan blue. Or automation of PK sampling with nanoliter by using echo technology allows increasing throughput of bioanalytical analysis for PK studies in Hoffmann La Roche. Also, another presentation of IGR has shown a complete robotic platform with 4 imagers (Micro from MDS) and 1 FACS totally automated from cell seeding to staining and reading.

It was a great pleasure also for us to present 4 different posters on our few last month works in cell imaging. This work was done in collaboration with Perkin Elmer on the Operetta machine. This machine allows us to take images in a 3D culture as shown in these posters: neuroprotection model for parkinson disease and oncology and performed also really nice images showed in these other posters: hepatotoxicity assay and genotoxicity assays.

Join us for the next ELRIGfr conference in Brussels, the 14th & 15th of october.

lab-processes-2015After Toulouse and Paris, the European Laboratory Research & Innovation Group (ELRIGfr) is happy to welcome you in Brussels for its next congress. It will be a great opportunity to exchange views and knowledge about laboratory automation, biobanking, imaging and to meet all key players in these fields.

You’re also invited to present your work at the posters sessions. Warning: the deadline to send your poster is septembrer the 15th. There is less than one week left to send us your abstract ! For more informations, please contact us at: info@elrigfr.com

The ELRIGfr conference will be located in the spacious and convenient Conference center of the Sheraton Hotel in the center of Brussels. The preprogramme of the event and the registration form can be viewed here.

We’re looking forward to seeing you in the capital of Europe !

Thomas Kirchhausen, Eric Betzig et al. publish an article in Sciences about SIM (structured illumination microscopy) and the observation of moving molecules inside cells. The incredible spatial and time resolution give the opportunity to see for exemple proteins which pass the cell membrane.

On the surface of a living cell at any given time, hundreds of tiny bubbles are popping into existence, surrounding and incorporating proteins, hormones, fats, and the occasional bacteria or virus. But until now the details of this activity were inferred – you couldn’t actually see it.

Li et. al., “Extended Resolution Structured Illumination Imaging of Endocytic and Cytoskeletal Dynamics,” Science.

Source : New Instrument Captures the Secret Lives of Cells – technologyreview.com

Aditional informations : Imaging Techniques Set a New Standard for Super-Resolution in Live Cells

Imaging on cells (HCA/HCS) and on animals are now possible. In this article, is reported both cell and animal imaging onAlpha-synuclein (αSYN) aggregation, a pivotal role in the Parkinson disease. Oligomerization of αSYN was monitored noninvasively with bioluminescence imaging in the mouse striatum and substantia nigra up to 8 months after injection.

The split firefly luciferase-αSYN complementation assay will increase our insight in the role of αSYN oligomers in synucleinopathies and opens new opportunities to evaluate potential αSYN-based neuroprotective therapies.

Curated from www.jneurosci.org

Leica Microsystems has launched Leica Application Suite X (LAS X), an easy-to-use imaging software for life sciences covering widefield, confocal and super-resolution platforms. LAS X introduces new features for image acquisition, processing and analysis while maintaining established principles of its predecessor software LAS AF, such as the workflow-based approach.

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