Aviv Regev at the Broad Institute, in Cambridge, uses fluidic systems to separate cells and submits them to detailed genetic analysis, at the rate of thousands per day. The goal is to build a comprehensive cellular human atlas, based on gene expression profiling.

The new technology works instead by cataloguing messenger RNA molecules inside a cell. These messages are the genetic material the nucleus sends out to make proteins. Linnarsson’s method attaches a unique molecular bar code to every RNA molecule in each cell. The result is a gene expression profile, amounting to a fingerprint of a cell that reflects its molecular activity rather than what it looks like.

NIH Grant has been awarded to Hudson Robotics and Johns Hopkins University for in vivo Studies. Cell imaging on in vivo studies on whole organism as zebrafish is a powerfull tool during drug discovery. Nevertheless, the throughput is really low to be used during preliminary drug discovery. Working on implementation of automated system for which a suitable in vivo assay can be developed in high throughput will save time and money while helping to streamline and speed up the drug discovery research process.

In vivo studies are done using a whole living organism. The HTS system being developed is termed the Automated Reporter Quantification in vivo (ARQiv) system. It is being created to bring high-throughput screening technology into the world of in vivo studies.

Curated from groundreport.com


Before the discovery and development of molecular biology, screening assays to find new drugs was performed on animals. Since the last decade with , assays on whole organisms come back in primary screening.

In this article, the author have identified 20 potential stimulators of beta-cell replication on transgenic line.

This study establishes a proof of principle for a high-throughput small molecule-screen for beta-cell proliferation in vivo, and identified compounds that stimulate beta-cell proliferation and regeneration.

Curated from www.plosone.org

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