It was the first time HCS Pharma participate at 35th symposium of the French society of genetic toxicology (SFTG) as a sponsor. The implication of the SFTG in the study and assessment of environmental genotoxic agents motive us to be a sponsor of this 35th event.

This year, topic focused on the trans generational genotoxic and epigenetic effects of environmental compounds. Two high level plenary session, presented by Francelyne Marano and Giacomo Cavalli from CNRS, has introduced clearly the different implications of trans generational genotoxicity and epigenetic effects. The role of germinal cells was clearly established in exposition to genotoxic compounds and we were pleased to see clinicians and lab scientists exchanged about this topic.

We also presented our last assay on micronucleus and gamma H2AX detection to the SFTG community. Thanks to the positive feedback received after the presentation, we are confident in the quality and robusteness of our automated genotoxicity assays.

If you want more information about this symposium, you can check the twitter account of the SFTG on @SFTGene

HCS Pharma is very pleased to be a sponsor of the 35th symposium of the french society of genetic toxicology (SFTG). This symposium attend in Marseille, the 26 and 27 Octobre at La Timone hospital. If you are interested in recent advances in genotoxicology, Pierre-Jean will present our new assay combining assessment of micronucleus and gH2AX/gH3, this afternoon at 14:00.

Find more informations on and feel free to contact us !

Automated Comet assay

The comet assay is a sensitive, well established technique for quantifying DNA damage in eukaryotic cells. Compatible with the detection of a wide range of DNA damaging agents, its principle consists in migration of fragmented DNA in an electrophoresis gel (damaged DNA forming the tail of the comet), while intact DNA moves at a slower rate (head of the comet). The percentage of fragmented DNA in the comet tail is a direct measure of DNA damage.

Micronucleus and H2AX assay

In response to clastogen DNA double strand breaks, H2AX is phosphorylated within minutes at Ser 139, and is then referred as γH2AX. Contrary to γH2AX, micronuclei are a late marker of damages either to DNA and be related to aneugens nor clastogens effect. By detecting both H2AX and micronuclei on our high content screening plateform, our assay provide a efficient tool to assess the genotoxicity properties of your compounds.

Carcinogenesis potential of new entity are really important to detect at the early stage of discovery. Cell imaging in HCA/HCS can allow to analysis micronuclei (MN) in a automation manner as we can see in this article on lymphocytes, which are suspension cells, analysed by laser scanning cytometry and high content analysis.

This fluorescent approach allowed clear identification of binucleated cells and detection of MN. Highcontent analysis was developed to further automatically score MN within mono-, tri- and tetra-nucleated cells and to determine the nuclear division index and nuclear circularity values. Importantly, it allows for co-detection of other biomarkers of interest within a single lymphocyte, and further development of this capability is anticipated.

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