There are many ways to look cells and inside components. At HCS Pharma we know well fluorescent microscopy in “classical” or “confocal” conditions but we are always interesting by others technologies and Cellular seismology is really promising !

In the video from CRCHUM & University of Montréal (Canada) we will see how to observe “cell quake” and why it is so useful to measure cell elasticity.

With simple equipment consisting of a standard microscope, micropipettes and a high-speed camera, the researchers developed a revolutionary method of observing, in real time, the displacements and forces present in mouse oocytes. According to Greg FitzHarris, a researcher at the CRCHUM, Université de Montréal professor and one of the project’s collaborators, “with this new cellular imaging method, we will be able to investigate new mechanisms involved in cell division during embryo formation”.

Source : Cellular seismology: Putting vibrations on the map

Ralf Jungmann, Ph.D., an alumnus of the Wyss Institute and currently a Professor of Biochemistry at the Ludwig Maximilian University (LMU) and the Max Planck Institute (MPI) in Germany and Wyss Institute Core Faculty member Peng Yin, Ph.D. have been developing DNA-PAINT, a powerful molecular imaging technology that involves transient DNA-DNA interactions to accurately localize fluorescent dyes with super-resolution.

Jungmann’s and Yin’s teams jointly work to the adaptation of DNA-PAINT to confocal microscopes. With this combination, visualization of cell internal becomes amazing!

“Our new approach, SDC-PAINT, integrates the versatile super-resolution capabilities of DNA-PAINT with the optical sectioning features of confocal microscopes. We thus created the means to explore the entire depth of a cell, and to visualize the molecules within it at the nanometer scale.”

Source: New Technique Visualizes Whole Cells at Super-Resolution

Sometime you have to stop and get back to basics of microscopy : optics, physics and photonics. At this time, the Carl Zeiss campus website is just a treasure ! You can spend hours in this website to find explanations on every thing you use every day.

For example, do you really know what is fluorescence microscopy and why we have to be careful in choosing dyes ? Do you imagine the complexity of Spinning Disk Confocal Microscopy we use in HCS Pharma (and why is far better than “classical” one) ? What are “tricks” to success in Live cell microscopy ? Yes, I like this website and, of course, I encourage you to discover it ! If you want to talk about microscopy and how to improve your pictures, don’t hesitate to contact me !

There are many resources on Internet to learn biology and understand used technics. iBiology is one of them, and a great one ! You can find informations on many domains from cell biology to synthetic biology and many talks on peripherical topics (impact on society, ethics, laws, etc.)
Close to our own skills, we advise you to look at courses on light microscopy (Basic Microscopy and In-depth Microscopy)

Biology’s mission is to convey, in the form of open-access free videos, the excitement of modern biology and the process by which scientific discoveries are made. Our aim is to let you meet the leading scientists in biology, so that you can find out how they think about scientific questions and conduct their research, and can get a sense of their personalities, opinions, and perspectives. We also seek to support educators who want to incorporate materials that illustrate the process and practice of science

Source: iBiology

We are happy to welcome a brand new startup in Rennes: Combo Microtech ! Combo Microtech is specialized in image acquisition. Their product will speed up the speed of acquisition for a “normal” microscope.

COMBO-microtech is a Start-up that will be created by O. Bouchareb and O. Chanteux during 2016 in order to commercialize POMM technology. The POMM technology was developed by O. Bouchareb as part of a maturation program funded by “SATT Ouest Valorisation” during 2015 at the IGDR. This project was supported by M. Tramier, leader of “R&D quantitave fluorescence microscopy” team.

Source: Rennes Atalante: Combo Microtech propose une solution intelligente de pilotage des microscopes

After with in high throughput called High Content Screening (HCS), super-resolved fluorescence microscopy was appeared. Eric Betzig, Stefan Hell, and William Moerner have won the 2014 in for their work on this technology. We can imagine that in the near future, we can perform  HCS with super-resolved microscopy. It will be a really challenge for lab automation suppliers, as Perkin, Molecular Device, GE healthcare, Thermofisher … to do it !!!

Along with advances in instrumentation and fluorescent probes, as well as in computational techniques, the work of Moerner and his co-Laureates has allowed scientists to observe and see individual single molecules in living cells in real time.

Curated from www.the-scientist.com

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