From 28 March to 1st April, the conference on DYRK1A and related kinases and human disease was held in Saint Malo. We have really enjoyed our venue as a sponsor of this conference. Thanks to the organizers, specially to Laurent Meijer and Pauline Demaison, we had a chance to present our services to the scientific community working on DYRKs protein. During 3 days, we have seen a lot of high quality scientific presentations from chemistry to the development of treatments for DYRKs related disease. We hope all these talks from international researchers will lead to scientific improvements in DIRK related disease.

The development of cellular models, especially in neurotoxicity, is an approach used to accelerate the development of new therapeutics for DYRK related disease. With our progress in 3D models, HCS Pharma hopes to provide new tools for researchers to better understand DYRKs protein,  and perhaps participate as a speakers at the next DYRK conference !

To see our assay in neurotoxicity just click here : http://hcs-pharma.com/services/toxicology/neurotox/

3D cell models are now attracting a huge interest from scientists working both on toxicity and pharmacology assay development. They are considered more relevant at mimicking the in vivo situation. However, phenotypic assays on these models can be challenging, and are at least more complex.

A team from Molecular Devices and Cellular Dynamics has worked on the “phenotypic characterization of toxic compound effects on liver spheroids derived from iPSC using confocal imaging an three-dimensional image analyis”. The results have been published September 2016 in Assay and Drug Development Technologies, and describe how the ImageXpress Micro Confocal High-Content Imaging System and MetaXpress High-Content Image and Analysis Software (Molecular Devices) were used to manage the phenotypic characterization.

Assuming that the approach may be extensible to more complex 3D systems, such as cultures containing multiple cell types (e.g., Kuppfer cells, fibroblasts, endothelial cells), they conclude 3D analysis would allow characterization of different cell populations and their roles in toxicity and liver injury.

Source : Phenotypic Characterization of Toxic Compound Effects on Liver Spheroids Derived from iPSC Using Confocal Imaging and Three-Dimensional Image Analysis

Since few years, the technology is more and more used to finaly create organ-on-a-chip and now body-on-chip with different organs. More and more academical units and collaborative projects allowed to succed to miniaturise different organs in different chambers on a chip connected with microfluidy. Lung, liver, kidney, heart, bone, bone marrow, adipose tissue, intestine, skin, blood vessels, blood-brain barrier are already exist on a chip. But the way is long and some problems are still not resolved as  the use of polydimethylsiloxane (PDMS), which absorbed hydrophobic drugs.

But can we imagine to have all organs of the body on a chip to test the safety of a drug in one in vitro experiment? This was the project of different partnairs and collaborations and one is in France in Compiegne:

“Une équipe française est active dans ce domaine. Basée à l’université de technologie de Compiègne, elle travaille avec le laboratoire franco-japonais LIMMS (CNRS université de Tokyo) à la mise au point d’un foie sur puce. En Europe, cinq partenaires, implantés en Suisse, Belgique, Allemagne et Royaume-Uni, collaborent à un projet de body-on-a-chip qui a reçu de l’UE un financement de 1,4.million d’euros. Une puce comprenant quatre types de cellules (foie, tumeur, muscle cardiaque, tissu nerveux) a été réalisée. Aux États-Unis, le projet de body-on-a-chip du MIT a reçu une subvention de 32 millions de dollars, le Wyss Institute une enveloppe de 37 millions.”

Can we imagine to replace the majority of animal testing and to be more and more predictive during the preclinical phases with all these new technologies (microfluidy, bioprinting,…). And by using cells coming from patients as IPS with using these new technologies, we will slowly but surely towards personalized medicine.

To know more on this project on body on a chip, go to this link (article in french): http://www.sciencesetavenir.fr/sante/e-sante/20151120.OBS9895/organes-artificiels-ces-puces-qui-miment-le-vivant.html

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