In Nature Methods from July, Xi Long and colleagues from the Howard Hughes Medicale Institute Janelia Research Campus (Ashburn, Virginia, USA) have developed a RNA fluorescence in situ hybridization (FISH) to study brain development and function, which require the expression of select genes at specific times in appropriate neurons.

We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).

Source : http://www.nature.com/nmeth/journal/v14/n7/full/nmeth.4309.html

Held in London from 4 to 6 April, in-cosmetics global has been a intense moment for HCS Pharma, to both encounter curent and potential partners, and to evaluate the curent and future trends in claims substantiation.

We thus had many opportunities to present how cellular imaging and image analysis can be used for anti-pollution assessment or microbiome equilibrium evaluation.

Of course, cellular imaging allows much more : at the sub-cellular level, we can provide you results for mRNA* visualisation and quantification, reactive oxygen species and lipid peroxydation semi-quantification, NFkB and NRF2 ttranslocation, and… Well, lets have a talk about what you need !

 

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