I was present at the last congress ELRIGfr in Brussels. It was a strong exchange place with interesting presentations around robotics. It is always really interesting to see examples of innovative automation which give really robust results. For example, Frank Gudermann from university of Bielefield in Germany showed that automation of culture cell with cell count by holographic picture gives more robust results as manual count by trypan blue. Or automation of PK sampling with nanoliter by using echo technology allows increasing throughput of bioanalytical analysis for PK studies in Hoffmann La Roche. Also, another presentation of IGR has shown a complete robotic platform with 4 imagers (Micro from MDS) and 1 FACS totally automated from cell seeding to staining and reading.

It was a great pleasure also for us to present 4 different posters on our few last month works in cell imaging. This work was done in collaboration with Perkin Elmer on the Operetta machine. This machine allows us to take images in a 3D culture as shown in these posters: neuroprotection model for parkinson disease and oncology and performed also really nice images showed in these other posters: hepatotoxicity assay and genotoxicity assays.

We are really pleased to announce that we have received the award of innovative poster for our Parkinson model and first results in 3D culture. This award recognizes our collaboration with two other startups to implement new innovative assays. These two other partnairs are CELENYS, which develops new plate for 3D culture which mimics the extracellular matrix, and QuantaCell, which develops new IT tools for image and results/statistics analysis (eg for 3D analysis and/or timelapse). With these two partnairs, HCS Pharma will seek to develop new assays in 3D culture by integrating extracelullar matrix to mimic as close to a healthy organ vs diseased organ. We hope to present new 3D validated assays during the next ELRIG session.

 

 

Nathalie and Julian were present during ELRIGfr event last week. They presented their company, their work and their collaborations. They have also presented two posters : the first one on a co-culture model of Parkinson and first results in 3 dimensions ; the other one on genotoxicity and steatosis assays on HepG2 cells. These next two to three months will be dedicated to validate these assays. New assays  will also be undertaken, like transporters assays, cholestasis or phospholipidosis, on HepG2 cells as well as on primary hepatotocytes. Genotoxicity assays are under validation on different cell lines as HepG2, SH-SY5Y, HeLa, HT29 cells. If you want more details, don’t hesitate to contact us.

First assays were performed these two last months on Parkinson disease cell model and we have obtained our first results on neuronal cell line culture and on co-culture of neuronal and macrophagic cells. A nice dose neurotoxicity effect was obtained with rotenone as Parkinson inducer. With these first results, we can now play with Columbus™ software to learn how it works for cell analysis.

HCS_neuro

%d bloggers like this: