“Manufacturers have developed super-corrected optics that allow co-localization studies with much more accuracy than before”, said Kevin Jia, senior marketing manager of Life Science at Olympus Scientific Solutions Americas in Waltham, Mass. “Also, throughput on our systems is much higher. Spectral imaging is vastly improved and live-cell imaging is possible with very low laser power.”

Working distance, wavelength ranges and other aspects of the optics now are tailored for specific applications to help confocal move from a fixed-cell technique to a highly sensitive, live-cell technique.

“We see confocal being combined with other imaging techniques,” Jia said. “For instance, we are looking at much higher-resolution and higher-contrast imaging through new superresolution technologies that are incorporated with spinning-disk or other confocal systems to create solutions providing fast, high resolution, high-contrast data.” One example is the Opera Phenix High Content Screening System from Perkin-Elmer, which is a microlens-enhanced Nipkow spinning disk confocal system that allows the capturing of confocal images from samples in multi-well plates or on slides at a very high throughput.

“Compared with conventional confocal laser scanning microscopes (CLSM), the Opera Phenix system allows unsupervised image acquisition, which can produce up to 400,000 images per day,” said Karin Boettcher, associate product manager for High Content Screening and Applications at PerkinElmer, Hamburg, Germany. “This far higher throughput, compared to conventional CLSM, allows screening of whole small molecule or siRNA libraries for a desired phenotypic effect.””

Sentences come from article on photonics.com internet site from Marie FREEBODY, Contributing editor.

To read the full article, go to this link: http://www.photonics.com/m/Article.aspx?AID=58209

Do you know what is phenotypic screening ? If you are here I think yes 🙂 But if you want to explain it easily, I strongly recommend the reading of Perkin Elmer white paper « Phenotypic Drug Discovery with High Content Screening ». It’s short (only 4 pages) but it contents main information to understand differences between « target based » and « phenotypic screening » approaches in drug discovery.

It explains also the importance of the technology side and the digital side of HCS and finally, give a good summarize of our vision :

“Today’s drug discovery strategies require candidate compounds to fail early and cheaply in the discovery stage, rather than late and expensively in the clinical phase. Testing compounds from the beginning in physiologically relevant model systems and leveraging the rich information available in image-based screens are ways to focus on those compounds that give rise to the right phenotypic changes without undesirable effects on the system. High Content Screening with highly detailed multiparametric assays in conjunction with modern machine-learning tools provide a promising way to achieve this goal.”

 Source : http://go.perkinelmer.com/Q315-WhitePaperEmailPKI-INF-LP?cid=4296

I was present at the last congress ELRIGfr in Brussels. It was a strong exchange place with interesting presentations around robotics. It is always really interesting to see examples of innovative automation which give really robust results. For example, Frank Gudermann from university of Bielefield in Germany showed that automation of culture cell with cell count by holographic picture gives more robust results as manual count by trypan blue. Or automation of PK sampling with nanoliter by using echo technology allows increasing throughput of bioanalytical analysis for PK studies in Hoffmann La Roche. Also, another presentation of IGR has shown a complete robotic platform with 4 imagers (Micro from MDS) and 1 FACS totally automated from cell seeding to staining and reading.

It was a great pleasure also for us to present 4 different posters on our few last month works in cell imaging. This work was done in collaboration with Perkin Elmer on the Operetta machine. This machine allows us to take images in a 3D culture as shown in these posters: neuroprotection model for parkinson disease and oncology and performed also really nice images showed in these other posters: hepatotoxicity assay and genotoxicity assays.

We are really pleased to work with Perkin Elmer group. We have now our own Columbus server  installed in our office for images visualisation and analysis. Thus we are now autonomous to work on our images and we have the possibility to give access to our clients to their images. We take time now to master control software and to play with it : a lot of work for Grégory, our IT guy, big data, image analysis specialist !

produce a lot of (pictures, meta-data, results, etc.) and it is not always easy to integrate and  them.

In this webinar, James Adams (Senior Regional Marketing Specialist for PerkinElmer) will focus on capabilities to integrate data from multiple systems.

The ultimate goal of “ Data” is to provide timely insight that is used to improve the effectiveness and efficiency of organizations. We live in a “data rich – information poor” world where access to data is not a problem but access to actionable information is.

Perkin Elmer and integromics worked together to create a new tools for HCS data analysis by integrating The High Content Profiler software into the visualisation tools already used by a lot of people in pharmaceutical industry: the TIBCO spotfire. This new solution will be presented for the first time next week in Barcelona, at the ELA2014 Congress. I will be really interested to know more on this new tool, and you?

source: 


%d bloggers like this: