I was really happy to be invited in SIBS 2017 in Zurich last week to present our work on 3D cell culture in phenotypic screening and our use of disruptive technologies in our process (augmented reality and virtual reality). I presented our collaborative work with Dr Karim Si-Tayeb, researcher from Institut du Thorax (CHOPIN project) on differentiation of iPS in hepatocytes in 3D culture for metabolic diseases screening. Karim has also the opportunity to present a poster on CHOPIN project with more information on iPS differentiation in hepatocytes and their use for phenotypic screening.

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The presentations during this event were really of interest with demonstration of the usage of new technologies as 3D culture, microfluidics, new microscopy technologies as light sheet microscopy. All these presentations showed that the development of cellular models for screening are made more and more complex, to be more and more physiological and relevant. As an example, Blandine Avignon from Roche has presented an epithelial barrier on a chip from Mimetas including 3D matrix and microfluidics. With this new device, they should follow the integrity of the barrier in fluorescence live cell microscopy.

I want to really thanks the organizers for this invitation and for this really nice and high scientific level event. See you in 2 years in Switzerland for the next SIBS event!

As you can see below (in french), our CDO Grégory MAUBON is not a biologist, he is a astrophysicist 🙂 Of course it doesn’t matter because he has to explore best ways to build 3D pictures and how to extract most relevant parameters … in high throughput of course !

Find the complete article in french on Connected Report

We want to thanks Connected MagDanielle Roméro and Thomas Gouritin for this interview !

We will be present at SIBS in Zurich, the international conference on Image-Based Screening. The program is plenty of interesting talks on “model systems in screening”, “advances in screening technology and assay”, or “images and data analysis”.

Furthermore, Nathalie, our CEO, is invited to present our activities on phenotypic screening on our innovative 3D culture assays. She will present you our activities, especially on our advances regarding the differentiation of patient-derived induced pluripotent stem cells for phenotypic screening in metabolic diseases. Furthermore, she will explain our vision on how new technologies, as virtual and augmented realities, deep learning and machine learning can be an advantage in phenotypic screening.

Nathalie hopes to see you in this congress to discuss on these topics! If you want to schedule a appointment, contact us.

Among the assays and technics we were busy to validate this summer is the histology slide imaging. HCS Pharma can now propose you to scan your histology slide on its automated MicroXLS microscope, with a magnification up to 600x. This service include the delivering of fully stitched images if required, that is even if your tissue or organoid or spheroid sample is far greater than the field of view when using a 60x objective for example, all the images willl be merged into one unique picture covering all the sample.

After taking the picture, an image analysis can be undertaken to quantitate the physiological responses you are looking for, with a subcellular resolution.

This service is now available on fluorescently stained tissue samples (up to 4 stains on the same sample).

H&E stained histology slide scanning coming soon !

Primary human hepatic spheroïd, 60X magnification (click for full size picture)

It’s always funny to see that the “old” maximum intensity projection is so used in picture reconstruction. Even if we all know artefacts created by this method, we often prefer a parameter free tool … In this article, a new way to do the 3D to 2D reduction is presented, the smooth manifold extraction, and it is compared to others one.

Three-dimensional fluorescence microscopy followed by image processing is routinely used to study biological objects at various scales such as cells and tissue. However, maximum intensity projection, the most broadly used rendering tool, extracts a discontinuous layer of voxels, obliviously creating important artifacts and possibly misleading interpretation. Here we propose smooth manifold extraction, an algorithm that produces a continuous focused 2D extraction from a 3D volume, hence preserving local spat

Source: Smooth 2D manifold extraction from 3D image stack | Nature Communications

Under the title of “High Content – Less Mess, More Mesh”, GEN (Genetic, Engeneering & Biotechnology news) has published this month an article about the advances that have made it possible to generate vaste datasets with decreasing costs and the need for comparing results and using informations collected from experiments on different cell types with different imaging systems.

“The biggest thing that needs to happen in the next few years is a more extensive interoperability of the information that is obtained from high-content screening and analysis,” insists Robert F. Murphy, Ph.D., the Ray and Stephanie Lane Professor of Computational Biology and professor of biological sciences, biomedical engineering, and machine learning at Carnegie Mellon University.

When descriptive features are used in the analysis of microscopy images, one of the challenges is to compare and integrate data across experiments, particularly when specific features captured using different experimental platforms may have different meanings for different investigators.

“One potential way to address this is to make the features interpretable,” suggests Dr. Murphy. “But that can be impossible if people use different microscopes, conditions, and objectives—and often different cells.”

Efforts to develop generative models of cellular organization and protein distribution from fluorescence microscopy images have been undertaken in Dr. Murphy’s laboratory. These efforts have led to the development of an open-source platform, the CellOrganizer project.

More details on GEN !

The first place has been won by Dr. Oscar Ruiz, of the University of Texas MD Anderson Cancer Center, for a confocal imaging of a 4 day-old zebrafish embryo.

4 day-old zebrafish

winner of the 2016 photomicrography competition

We invite you to spend your next break to explore these breathtaking images on the smallworld Nikon website. 🙂

We just begin a test period of the Operetta “High Content Imaging System” from Perkin Elmer. First impressions are very good, especially using confocal option for 3D imaging. As you can see below, neurites growth is quite easy to observe around and inside a spheroid of SH-SY5Y cells. To properly count it is more complicated for the moment but we progress in the mastering of “Harmony” and “Columbus” softwares, as for “Acapella” scripts. Our next challenge is to build comprehensive 3D visualisation with Volocity software.

It was a pleasure for us to welcome Antoine at HCS Pharma for a 6 week internship focused on statistics interpretation improvement. He worked particularly on cells culture homogeneity in a 96 wells plate, and effect of cells spatial localisation in a well on results interpretation. Now, after a well deserved holiday, he will prepare a M2 about “public statistics” in Rennes 1 university.

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