During these last two weeks, we took control of our new robotic platform and validated the pipetting volumes and the entire process by testing it with a few plates. Some steps are still to be improved, such as the scheduling of the plates. The first screening with a few plates was on hepatotoxicity assay on SCREEN-WELL® Hepatotoxicity library from ENZO (238 compounds) in one concentration.

After improving our process, we will test this library in dose effect in addition to the Prestwick library. We will also perform neurotoxicity assay in a screening mode on internal and Prestwick library. These assays will allow us to validate all our process to cell culture until data analysis in addition to exemplify data on our toxicity assays.

In-Cosmetics Paris, April 12 to 14

Come and visit us at in-cosmetics, the meeting of the major groups of cosmetics, Porte de Versailles in Paris.

We will exhibit Stand P43 within the Cosmetic Valley in partnership with Bretagne Commerce International.

We will be happy to welcome and present you our services in phenotypic screening and cell imaging.

Do you know what is phenotypic screening ? If you are here I think yes 🙂 But if you want to explain it easily, I strongly recommend the reading of Perkin Elmer white paper « Phenotypic Drug Discovery with High Content Screening ». It’s short (only 4 pages) but it contents main information to understand differences between « target based » and « phenotypic screening » approaches in drug discovery.

It explains also the importance of the technology side and the digital side of HCS and finally, give a good summarize of our vision :

“Today’s drug discovery strategies require candidate compounds to fail early and cheaply in the discovery stage, rather than late and expensively in the clinical phase. Testing compounds from the beginning in physiologically relevant model systems and leveraging the rich information available in image-based screens are ways to focus on those compounds that give rise to the right phenotypic changes without undesirable effects on the system. High Content Screening with highly detailed multiparametric assays in conjunction with modern machine-learning tools provide a promising way to achieve this goal.”

 Source : http://go.perkinelmer.com/Q315-WhitePaperEmailPKI-INF-LP?cid=4296

HCA/HCS assays are now realised on cell models which are more and more complex as 3D and/or co-culture. And to be more relevant, imaging assay in low/medium troughput can also be performed on whole organism as zebrafish. Here is an interesting video from Department of Biological Sciences, University of Notre Dame, explaining how to do imaging on zebrafish embryos.

The zebrafish is an excellent experimental organism to study vertebrate developmental processes and model human disease. Here, we describe a protocol on how to perform a manual high-throughput chemical screen in zebrafish embryos with a whole-mount hybridization (WISH) read-out.

Curated from www.jove.com

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