There are several products today for 3D cell culture and many could be considered as “hydrogels”. In this short article, we explain differences between BIOMIMESYS® and hydrogels to help you to choose the right product for your very specific needs.

In 3D cell cultures, many hydrogels could be used like the well-known Matrigel®, or Geltrex™. Such basement membrande-derived hydrogels have specific compositions and physico-chemical, biochemical properties, and a defined range of usages. Below, you can find the main differences between them and BIOMIMESYS®, for you to choose the product you need.

Of course if you want more specific details, please contact us.

Basement membrane-derived hydrogelBIOMIMESYS®
Biochemical featuresThermosensitive hydrogelHydroscaffold™ with a dual behavior (hydrogel + solid scaffold), as the native ECM (extracellular matrix)
SourceSecreted extracellular matrix proteins, purified from murine Engelbreth-Holm-Swarm (EHS) tumor cellsNative ECM components: hyaluronic acid secreted by bacteria + collagens from rat tail or of human origin, adhesion peptides or proteins (RGDS peptides) and other ECM components, upon request
Composition & reproducibility (batch-to-batch)Includes laminin, collagen IV, heparin sulfate proteoglycans, and entactin/nidogen + growth factors.
The composition varies from one batch to another (lot-specific protein concentration).
Composition depends on the reference, since BIOMIMESYS is an organ/tissue-specific matrix (BIOMIMESYS Liver, Adipose Tissue, Brain, and Oncology (generic). Since it is chemically mastered, the composition is stable from one lot to another.
TransportationOn dry iceAt room temperature
HandlingViscous solution that needs to be slowly thawed on ice, aliquoted and then handled at 4°C. Need for RT to polymerize.Ready-to-use in your cell culture vessel => Minimal handling: no need to aliquot, polimerize…
StorageAt -20°CAt 4°C
Porosity<10 µm1Average porosity is around 100-150 µM: cells diffuse in the matrix
How the cells are in there?Cells are encapsulated in the hydrogel.Cells diffuse in the matrix since it is porous, then they can nest and develop inside. The high porosity facilitates the diffusion of gaz and nutrients.
Optical propertiesTransparentTranslucent
Format of the cell culture vesselAll formats, but you do it yourselfAll formats, but  we cast BIOMIMESYS for you
Long-term 3D cell cultureNeed to add some hydrogel along time in the culture medium, since not stable along timeBIOMIMESYS is stable along time (nothing to add)
StiffnessCannot be adjusted over a large range (maximum 300 Pa2)Controlled stiffness, possibility to choose different elastic moduli (from 100 Pa to 16 kPa, depending on your organ/tissue of interest). Can be tailored upon request.
Possibility to take out the matrix together with the cells from the plateNo, they are stuck there until the end of the experiment.Yes, you can take the matrix (with cells within) with a clamp and move it (for example to put it on a glass slide…)
BioactivityPresence of tumoral growth factors (decreased in growth-factor-reduced versions)No growth factors, except the ones your cells will secrete within or that you add to your culture (you completely master this aspect).

1 Zhang S, Altman M, Aggeli A, Boden N. Self-assembling Peptide Systems in Biology and Biomedical Engineering. In 2007. p. 343‑60.


Please feel free to contact us here for any question about BIOMIMESYS, we will be able to propose to you the right product for your needs!

Grégory MAUBON

Grégory MAUBON is Chief Data Officer and digital coordinator at HCS Pharma, a biotech startup focused in high content screening and complex diseases. He manages IT missions and leads digital usages linked to company needs. He is also a Augmented Reality Evangelist (presenter and lecturer) since 2008, where he created and founded in 2010 RA'pro (the augmented reality promotion association). He helped many companies (in several domains) to define precisely their augmented reality needs and supported them in the implementation.


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