BIOMIMESYS® hydroscaffold is porous, and the porosity depends on the reference:. Commercialized BIOMIMESYS plates have an average porosity of 100-200 µm. For specific applications, we can modulate the porosity on demand.
BIOMIMESYS® hydroscaffold plates are sterilized and put under vacuum: they are delivered dehydrated. It is recommended to store the plates at 4°C upon receipt. BIOMIMESYS® hydroscaffolds are stable for 12 months at 4°C, if the packaging is intact.
BIOMIMESYS® hydroscaffold is the only ready-to-use and organ/tissue-specific technology based on hyaluronic acid (HA) and different naturally occurring extracellular matrix components. With BIOMIMESYS® hydroscaffold we can better mimic the microenvironment of any organ, healthy or pathological (composition and mechanical properties). It is compatible with all classic analytical methods and microscopy.
Yes, for a first try we suggest that you order a 96-well plate containing 24 hydroscaffolds.
All commercialized plates are in the 96-well format. For specific formats (e.g. 6-, 12-, 24- and 48-well plates), use this form to explain your need and we will get back to you in a day.
Since BIOMIMESYS® is a versatile technology, we can customize the plates on demand in terms of composition, porosity, stiffness and format. You can use this dedicated form to explain your needs and, of course, we will be happy to help you in your design.
To be aware of new BIOMIMESYS® hydroscaffolds, you are invited to subscribe to our newsletter. We would be glad to welcome you in BIOMIMESYS® R&D program, feel free to contact us.
You can cultivate cell lines, primary cells, stem cells and induced pluripotent stem cells (iPSCs) in BIOMIMESYS®. Please contact us if you need help to choose between our different products or if you need a specific matrix.
BIOMIMESYS® plates should be taken out from the fridge (4°C) for them to reach room temperature, and unpacked just before seeding. For general applications, the cells should be resuspended within a small volume of medium, carefully loaded at the surface in the middle of each hydroscaffold without touching the matrix Read more…
As soon as seeded, cells will enter the pores of the matrix. With time your cells should colonize the hydroscaffold (under the form of spheroids for example). Since the hydroscaffold is composed of naturally occurring extracellular matrix components (Hyaluronic Acid, collagens), cells will be able to remodel it as they Read more…
All hydroscaffolds from one plate should be used at the same time, otherwise the remaining ones will get moistured and lose their properties. If you do not need 96 hydroscaffolds, you can order the plates containing only 24 matrices.
All usual analysis techniques tested so far have proven to be compatible with BIOMIMESYS® (microscopy, immunocytochemistry, qPCR, Western blot, ELISA, histological techniques, electron microscopy, flow cytometry, etc.). If you need any help, please refer to the validated protocols or contact us to meet your specific needs.
You can find available protocols on this page : Protocols. If you need more information or help to use BIOMIMESYS®, please contact us !
Yes, a specific protocol has been developed to retrieve cells/spheroids : Retrieval of spheroids or cells BIOMIMESYS® Protocol
Yes. We use it internally with an automated platform, and have tested it with INTEGRA pipetting system ViaFlo (please refer to our common application note: Application note)
First, take a look at the Starting Guide Protocol and Tutorials page. For any questions, please feel free to contact us!
Thanks to the physical properties of BIOMIMESYS hydroscaffold (solid and elastic), the in vivo implantation is possible without dissolution of the hydroscaffold.
We recommend the use of BIOMIMESYS® Oncology or to test the corresponding organ specific matrix (eg. HepG2 BIOMIMESYS® Liver )
We recommend the use of BIOMIMESYS® Brain hydroscaffold, for neuronal cell cultures
BIOMIMESYS hydroscaffold is stable for 12 months at 4° C, from the date of release which is indicated on the CoA of the product (list of CoA). We also guarantee reproducibility from batch to batch (another advantage of BIOMIMESYS technology).
Yes, you can observe bubbles following cell seeding. They disappear within a few days, after renewing the medium.
The physicochemical properties of BIOMIMESYS® hydroscaffold allow immunohistochemistry (fixation, freezing and microtome). For more information look at “Immunostaining in BIOMIMESYS® hydroscaffolds™“