Human liver organoids and disease models, it’s time to use a more realistic ECM

The current hiPSC-derived models are adapted to reproduce diseases of genetic origin in two dimensions and have allowed in recent years to model diseases by using hepatocyte-like-cells (HCL) such as: Alpha1-antitrypsin (A1AT) deficiency (Tafaleng et al., 2015) Familial transthyretin-related amyloidosis (ATTR) (Isono et al., 2014) Glycogen storage deficiency (Satoh et Read more…

Extracellular Matrix Modifications in Lung Cancer Initiation, Progression and Propagation

The Extracellular Matrix (ECM) is a major component of all the organs. This dynamic structure is involved not only in cell adhesion, but also in physiological processes, including cell differentiation, migration, and maturation, tissue homeostasis, biochemical signal retention and mediation, and growth factors’ retention. Moreover, the ECM can be abnormally Read more…

Extracellular matrix modifications are involved in cancer initiation, progression and propagation

The extracellular matrix (ECM) is the non-cellular component of tissue. Its is mainly composed of structural and adhesion molecules, such as hyaluronic acid, collagene, proteoglycans, fibronectin, elastin, although its specific composition and structure vary between different tissues. It is a dynamic structure, which is involved not only in the cell Read more…

A single procedure to generate functional hiPSCs-derived liver organoids -Towards an innovative tool suitable for drug screening

The current hiPSC-derived models are adapted to reproduce diseases of genetic origin. However, the current two-dimensional HLC (hepatocyte like-cells) model has an immature liver phenotype that is more is closer to a perinatal liver. Furthermore, it does not take into account the complexity of the tissues and the extracellular environment Read more…

Poster – A single procedure to generate functional hiPSCs-derived liver organoids -Towards an innovative tool suitable for drug screening

Ask for high quality link by putting your email below We previously showed that human pluripotent stem cells (hiPSCs) provide a suitable model to study metabolic diseases upon hepatocyte-like cell (HLC) differentiation. In particular, HLCs have been used to model cholesterol metabolism regulation, by mimicking the main disease features in Read more…

Importance of the liver extracellular matrix in the development of NASH/ASH

The extracellular matrix (ECM) is a non-cellular component of tissues and organs. It is mainly composed of structural elements (proteoglycans collagens, elastin) and cell adhesion proteins such as fibronectin and laminins. The ECM forms a complex structure with physical properties (elasticity, tensile and compression strength), which provides physical support for Read more…

Importance of the microenvironment in cerebral in vitro models for phenotypic screening (Abstract of Veronique’s thesis)

About 90% of drug candidates fail in clinical trials, for efficacy- and toxicity-related reasons, which often involve the Central Nervous System (CNS). This high failure rate highlights a lack of relevance in experimental models used upstream, including human in vitro models. Indeed, they do not take into account the complexity Read more…

Collaboration between “Institut du thorax” and HCS Pharma: improving the management of hypercholesterolemia using a relevant and innovative organoid-based in vitro liver model

Launched in 2016, the adventure is coming to an end after 5 years of work carried out within the RHU CHOPIN (CHOlesterol Personalized INnovation), and with a strong collaboration between HCS Pharma and l’Institut du thorax. This collaborative project of national scope was led by Professor Bertrand Cariou, director of Read more…