Our project director Elodie is participating to EUSAAT congress in Linz (Austria) this week, the European Congress on Alternatives to Animal Testing. She will have the pleasure to present our work aiming at bridging the gap between in vivo and in vitro situations by taking into account the extracellular matrix Read more…
Presentation in english Environ 90 % des candidats médicaments échouent aux essais cliniques, notamment en neurologie, faute d’efficacité. Cela met en évidence un manque de pertinence dans les modèles précliniques, y compris les modèles in vitro, qui ne prennent pas en compte le microenvironnement, composé par les cellules gliales et Read more…
Présentation en français About 90% of drug-candidates failed in clinical trials, in particular in neurology, due to a lack of efficacy. That highlights a lack of relevance in preclinical models, including in vitro models, which do not take into account the microenvironment, composed by glial cells and the Extracellular Matrix Read more…
There are several products today for 3D cell culture and many could be considered as “hydrogels”. In this short article, we explain differences between BIOMIMESYS® and hydrogels to help you to choose the right product for your very specific needs. In 3D cell cultures, many hydrogels could be used like Read more…
We are very pleased to talk with Pr Robert-Alain Toillon from University of Lille about his uses of BIOMIMESYS® technology in oncology and more specificaly on breast cancer. English is below Je m’appelle Robert-Alain Toillon je travaille au laboratoire Canther dans l’institut OncoLille sur le cancer du sein et plus Read more…
We previously showed that human pluripotent stem cells (hiPSCs) provide a suitable model to studymetabolic diseases upon hepatocyte-like cell (HLC) differentiation. With a non-invasive approach, hiPSCs can be generated from urine samples of patients and HLCs have been used to model cholesterol metabolism regulation, by the study of LDLR- and Read more…
Aim: About 90% of drug-candidates failed in clinical trials, in particular in neurology, due to a lack of efficacy. That highlights a lack of relevance in preclinical models, including in vitro models, which do not take into account the microenvironment, composed by glial cells and the Extracellular Matrix (ECM). The Read more…
Solid tumors are associated with a modified microenvironment, in particular an enhanced collagen content and increased stiffness at the level of the extracellular matrix (ECM). These ECM changes are now recognized as important drivers of cancer initiation, progression, and propagation, and also impact the responses of cancerous cells to treatments. Read more…
To treat brain disease is a challeging purpose, since the brain is protected by a barrier made by of tightly-joined endothelial cells of the cerebral microvessels, called the blood–brain barrier (BBB). To improve this route to reach the central nervous system, several strategies have been developed. Among them, researchers try Read more…
We previously showed that human pluripotent stem cells (hiPSCs) provide a suitable model to study metabolic diseases upon hepatocyte-like cell (HLC) differentiation. In particular, HLCs have been used to model cholesterol metabolism regulation, by mimicking the main disease features in vitro. Human iPSCs can be generated from urine samples of Read more…
In HCS Pharma we are specialized in 3D cell culture and we have developed high skills in 3D imaging thanks to the help of our partner Molecular Devices. We are used to using powerful ImageXpress Micro Confocal Systems to investigated 3D cells morphology in HCS context from fluorescence pictures. The Read more…
HCS Pharma était présent au Workshop OoC pour une création et une structuration de la filière, organisé par Biovalley à l’Institut Pierre-Gilles de Gennes (Paris). Nous sommes très heureux d’avoir pu sponsoriser l’événement, mais aussi tout simplement d’y avoir participé – en présentiel – et de pouvoir discuter avec des chercheurs Read more…
Ask for high quality link by putting your email below How to make in vitro models predictive of in vivo conditions? By taking into account the 3D cellular organization of in vivo tissues By including the cellular and matricial microenvironments with BIOMIMESYS® By using OoC systems for dynamic in vivo-like Read more…
A relevant liver in vitro model should display CYP inducibility that mimics in vivo metabolic responsiveness to known modulators. This response of CYPs to induction is a critical parameter for predicting adverse drug reactions.
Maintaining the basal characteristics of primary hepatocytes and HepG2 allows performing metabolic studies. The functional instability of hepatocytes in 2D culture results in a rapid decrease in specific metabolic pathways (Figure 1). Cell culture in BIOMIMESYS® Liver shows higher metabolic characteristics of hepatocytes compared to a culture in 2D, such Read more…
Solid tumors are associated with a modified microenvironment, in particular an enhanced collagen content and increased stiffness at the level of the extracellular matrix (ECM). These ECM changes are now recognized as important drivers of cancer initiation and progression, and also impact the responses of cancerous cells to treatments. Made Read more…
BIOMIMESYS® Liver recreates the complex cellular microenvironment of the liver to extend longevity and functionality of hepatocytes in culture: The life of human primary hepatocytes grown in a 2D sandwich is low, 3-10 days (1) and is accompanied by dedifferentiation, leading to the loss of their functions. In 2D culture, Read more…
Hepatotoxicity testing in animals predicts only 50% of liver damage observed in clinical context, in part, because of difference in inter-species hepatic metabolism (1). Therefore, a relevant in vitro model mimicking the micro-environment of the human liver for maintaining and promoting hepatocyte functions, is needed for a better hepatotoxicity prediction Read more…
Pre-adipocytes differentiate into adipocytes through several phases: differentiation and maturation, where they are then completely functional (lipogenesis / lipolysis).
Retinoic acid and Caffeine are reference molecules known for their inhibitory activity on lipogenesis. Retinoic acid, a derivative of vitamin A, inhibits adipogenesis by blocking adipogenic factors such as PPARγ2 and C / EBPα, via the Retinoic acid receptor (RAR) ligand (1). Caffeine, a non-selective adenosine antagonist receptor, is used Read more…
The morphological changes in cells during differentiation are accompanied by a profound change at the molecular level. In vivo, the adipocyte differentiation process includes early and late events due to differential gene regulation. The appearance of these markers is a sign of differentiation of pre-adipocytes and hence the development of Read more…
Triglycerides (TGs) are an efficient form of fatty acids to accumulate as energy reservoirs or as building blocks for membrane lipid synthesis. In adipose tissue, TGs are continuously synthesized during lipogenesis. In order to measure the lipogenesis activity of 3T3-L1 and mature human adipocytes, Adipored™ staining and Perilipin immunocytochemistry were Read more…
Adipocytes have a characteristic round morphology in vivo, due to the production of one large lipid vesicles, created by lipogenesis (1). In conventional cell culture, adipocytes cannot acquire this physiological morphology.