Scanning Electron Microscopy (EM) has been an indispensable tool for Biological sciences. A great challenge was propelled by the need to find methods to better preserve and analyze structures at a state closest to the native state. With BIOMIMESYS® hydroscaffold technology, we have developed a new method to use SEM to analyze the morphology of cells, spheroids or organoids. And thanks to our protocol we analyzed the structure of our hydroscaffold like its porosity, the formation of interpenetrated networks between Collagens I and IV and crosslinking Hyaluronic acid (HA).

Thanks to our innovative protocol, we can maintain BIOMIMESYS hydroscaffold structure and cell morphology. As an example using the hepatic cell line HepG2, an organization of the spheroid and the interaction of the spheroid matrix are observed.

Knowing that primary liver cells (primary Human Hepatocytes) are fragile cells. The same protocol makes it possible to see the organization of the aggregates and to highlight the interactions with the matrix.

The last example shows the structure of a liver organoid derivatives from iPSCs. We observed the morphological organization of an organoid and its anchoring on the BIOMIMESYS hydroscaffold.

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