Micronucleus analysis by laser scanning cytometry

Carcinogenesis potential of new entity are really important to detect at the early stage of discovery. Cell imaging in HCA/HCS can allow to analysis micronuclei (MN) in a automation manner as we can see in this article on lymphocytes, which are suspension cells, analysed by laser scanning cytometry and high content analysis.

This fluorescent approach allowed clear identification of binucleated cells and detection of MN. Highcontent analysis was developed to further automatically score MN within mono-, tri- and tetra-nucleated cells and to determine the nuclear division index and nuclear circularity values. Importantly, it allows for co-detection of other biomarkers of interest within a single lymphocyte, and further development of this capability is anticipated.