More and more articles show the advantages of #3d culture compared to 2D in #oncology field. This article describe how to do high throughput using 3D culture by rapid size profiling analysis over time on tumor spheroids.
“Tumor size is the most frequently used in vivo endpoint when assessing antitumor efficacy in animal xenograft models, whereas proliferation is the more typically evaluated growth endpoint in vitro using two-dimensional (2D) monolayer cultures. Such 2D in vitro assays frequently fail to correlate with in vivo observations, owing to the inability of 2D cultures to recapitulate the native tumor microenvironment described above. Three-dimensional (3D) tumor microtissues, or multicellular tumor spheroids, are considered a more representative, organotypic model for assessment of tumor growth. They contain layers of cells that exhibit more in vivo-like size- and gradient-dependent proliferation and viability profiles.”
To know more about this article, follow this link: http://www.genengnews.com/gen-articles/phenotypic-drug-discovery-in-3d/5303/