Photobleaching, a great advantage in super-multiplexed fluorescence microscopy!

In a recent article, Dr Antony Orth from the Australian Research Council’s, Centre of Excellence for Nanoscale BioPhotonics (CNBP)  explains how to use the photobleaching phenomenon to increase the potential of fluorescence microscopy. The BAMM technique (bleaching-assisted multichannel microscopy) takes advantage of the rate of photobleaching to identify a fluorophore (instead of a specific emission wavelength). Therefore it makes possible the use of several fluorescent molecules that emit in the same wavelength range simultaneously in a sample! Dr Antony Orth shows that it’s a way to use from 8 to 12 fluorophores at the same time and extract much more information than ever…

BAMM technique. Credit: CNBP

Sources :

• • Super-multiplexed fluorescence microscopy
  • Fluorescence microscopy gets the BAMM treatment
  • Super-multiplexed fluorescence microscopy via photostability contrast