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Poster presented during the SLAS event in San Diego on the 27th and 28th of February 2023.
Title: BIOMIMESYS® Hydroscaffold, a ready-to-use organ-specific Extracellular Matrix for Relevant Phenotypic Screening
Dr. Nathalie Maubon*, Dr. Véronique De Conto, Dr. Méryl Roudaut, Dr Alejandra Mogrovejo – Valdivia, Julien Leriche, Vaihere Cheung and Dr Elodie Vandenhaute
The extracellular matrix (ECM) is present in all tissues and is a master regulator of cellular behavior and phenotype: it influences the anchorage of the cells, acts as a migration barrier and track, signal reservoir, low-affinity co-receptor, signal presenter and is a source of biochemical forces. The ECM in each tissue/organ is characterized by a specific composition, and by biochemical and biophysical properties. Importantly, ECM features are modified in different types of disease, like cancers and fibrotic conditions. Given the importance of the matricial microenvironment in maintaining tissue homeostasis and proper intercellular communications, we aim at integrating the ECM parameter in in vitro models to better mimic the in vivo complexity.
For this purpose, we have developed 3D cellular models using BIOMIMESYS®, a patented green chemistry hydroscaffold™ for 3D cell culture. This matrix exhibits unique dual properties, unifying hydrogel and solid scaffold features in a single matrix. This highly reproducible matrix is suitable for long-term 3D cell culture. BIOMIMESYS® is based on Hyaluronic Acid (HA), a major component of the ECM, biofunctionalized with other ECM components (collagens, adhesion proteins or peptides) depending on the organ/tissue of interest. Moreover, the stiffness is also modulated to fit with the healthy or pathological ECM to reproduce (elastic modulus from 0.1 to 16 kPa).
In this poster, we will exemplify the importance of the matricial environment with the improvement of cell longevity and functionality and with successful differentiation of human pluripotent stem cells (hiPSCs) into mature and functional 3D liver organoids within BIOMIMESYS®. Moreover, we will present some results in oncology field and how we can model the tumoral ECM with a representative range of stiffnesses (1, 8 and 16 kPa) in vitro.
By better mimicking the ECM microenvironment, the next generation of 3D cell culture models should help to model in vivo complexity with more relevance, and therefore to discover new effective therapies against diseases like cancers. Our 3D in vitro models, by taking into account the matricial microenvironment, aim at highly improving phenotypic screening for a better prediction of human outcomes.
Keyword: Extra-Cellular Matrix, 3D cell culture, Phenotypic Screening, High Content Screening, HCS