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Hepatocytes in primary cultures are still the gold standard in the pharmaceutical industry ; however they are expensive and have a very short lifespan with a progressive loss of specific functions over time. The dipolar aprotic solvent, dimethyl sulfoxide (DMSO) is wildly used to stabilize the liver specific function of human hepatocytes in vitro. It is also used to potentialize the differentiation of the human HepaRG-hepatocytes-like cells that share functional characteristics with human hepatocytes. Besides its free radicals scavenging properties that contribute to hepatoprotection, DMSO has been shown to interfere with apoptosis signalling pathways. To avoid this disadvantage, we have analysed the functional properties of freshly isolated human hepatocytes (FIH) and HepaRG-hepatocyte-like cells in 3D cultures performed using Ultra Low Attachment Technology (ULA) plates.

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Grégory MAUBON

Grégory MAUBON is Chief Data Officer and digital coordinator at HCS Pharma, a biotech startup focused in high content screening and complex diseases. He manages IT missions and leads digital usages linked to company needs. He is also a Augmented Reality Evangelist (presenter and lecturer) since 2008, where he created and founded in 2010 RA'pro (the augmented reality promotion association). He helped many companies (in several domains) to define precisely their augmented reality needs and supported them in the implementation.

1 Comment

Our feedback from ESTIV 2018 | HCS Pharma · November 15, 2018 at 5:28 pm

[…] We were also present in the poster section to present our works on the characterisation of 3D cultur…. This work was made in collaboration with NUMECAN, from Rennes University, FRANCE. […]

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