Early detection of toxic events induced by drug cantidats is mandatory in order to avoid late attrition in the process of R&D. Steatosis is an accumulation of triglicerids within the liver cells and may lead steatohepatiitis. Phospholipidosis is a disruption of the phospholipidases activity, either by direct inhibition or through drug-phospholipid complexes that cannot be broken by them, leading to intracellular phospholipid accumulation. Although not a toxic event by itself, phospholipidosis predict drug and metabolite accumulation that may provoke toxicity within the liver or kidney for example. Drug induced steatosis and phospholipidosis can be quantified by High Content Screening.
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