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To find anti-cancer drugs, different cellular modifications can be looked at: cell death effect on proliferating cells either in 2D culture or on spheroids (3D culture), anti-mitotic, anti-migration/invasion, anti-angiogenesis effects… Different assays can be performed to follow these different effects. High content screening is a multi-parametric technology allowing searching for poly-effects drugs.

In this poster, several well known compounds were tested in 3 different assays: cytotoxicity in 2D culture, cytotoxicity in spheroids (3D culture) and scratch assay (also named wound healing). These 3 models were automated in 96-well plates.

View and download on Slideshare (low quality) :
https://fr.slideshare.net/hcspharma/high-content-screening-of-automated-wound-healing-and-cytotoxicity-assays-in-2d-and-3d-culture


Grégory MAUBON

Grégory MAUBON is Chief Data Officer and digital coordinator at HCS Pharma, a biotech startup focused in high content screening and complex diseases. He manages IT missions and leads digital usages linked to company needs. He is also a Augmented Reality Evangelist (presenter and lecturer) since 2008, where he created www.augmented-reality.fr and founded in 2010 RA'pro (the augmented reality promotion association). He helped many companies (in several domains) to define precisely their augmented reality needs and supported them in the implementation.

3 Comments

Our feedback of lab automation congress of ELRIGfr in Bruxelles | HCS-pharma · November 17, 2015 at 8:17 am

[…] images in a 3D culture as shown in these posters: neuroprotection model for parkinson disease and oncology and performed also really nice images showed in these other posters: hepatotoxicity assay and […]

Elrig » Event in BrusselsElrig · December 2, 2015 at 11:23 am

[…] images in a 3D culture as shown in these posters: neuroprotection model for parkinson disease and oncology and performed also really nice images showed in these other posters: hepatotoxicity assay and […]

ELRIGfr » Retour sur notre dernier événement à BruxellesELRIGfr · April 19, 2016 at 6:38 pm

[…] in a 3D culture as shown in these posters: neuroprotection model for parkinson disease and oncology and performed also really nice images showed in these other posters: hepatotoxicity […]

Our feedback of lab automation congress of ELRIGfr in Bruxelles | HCS Pharma · December 14, 2020 at 6:08 pm

[…] images in a 3D culture as shown in these posters: neuroprotection model for parkinson disease and oncology and performed also really nice images showed in these other posters: hepatotoxicity assay and […]

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