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Quantifying DNA damage is mandatory to assess potential adverse effects of candidate, drugs, molecules or extracts developed in dermo-cosmetic industry. Different assays can be performed to detect primary DNA damages, such as γH2AX or the single cell gel electrophoresis also known as the comet assay. The High Content Imaging technology was used as a valuable tool for screening in early discovery phase.
Four non genotoxic compounds and twelve genotoxic compounds from the ECVAM list I or II were selected to be tested on different cell models. Preliminary results on Mitomycine C, 4 Nitroquinoline-N-oxide and D-mannitol are shown here.
View and download on Slideshare (low quality) : https://fr.slideshare.net/hcspharma/use-of-automated-high-content-analysis-applied-to-assessment-of-primary-dna-damages-with-h2ax-and-comet-assays