Screen Approaching using zebrafish embryos

HCA/HCS assays are now realised on cell models which are more and more complex as 3D and/or co-culture. And to be more relevant, imaging assay in low/medium troughput can also be performed on whole organism as zebrafish. Here is an interesting video from Department of Biological Sciences, University of Notre Dame, explaining how to do imaging on zebrafish embryos.

The zebrafish is an excellent experimental organism to study vertebrate developmental processes and model human disease. Here, we describe a protocol on how to perform a manual high-throughput chemical screen in zebrafish embryos with a whole-mount hybridization (WISH) read-out.