Glutathione (GSH) is the major antioxidant endogenous peptide, which scavenges reactive oxygen species (ROS) including H2O2. GSH is also involved in detoxifying processes by binding heavy metals, forming water soluble conjugates. Endogenous GSH pool reduction is a cause of oxidative stress damages associated with mitochondrial deterioration. Monochlorobimane reacts with GSH to form a highly fluorescent dye.

Figure 1 : Labbeling of intra cellular glutathione.

Quantifying GSH pool is a marker of anti oxidative properties of compounds. To defined is compounds enhance a curative nor protective effetc, we compared the GSH pool with an oxidative species inducer Menadione (MEN), and GSH inducer N-Acetyl-Cystein (NAC).

Figure 2 : Primary differentiated keratinocytes exposed to NAC (N-acetyl-cystein) and MEN (menadione).

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