The skin microbiota refers to the microorganisms living on the skin, on the surface of the epidermis, but also in the dermis and the hypodermis. On 1 cm2 of human skin, up to a billion microorganisms are residing, including bacteria, fungi, mites, and viruses.

In vitro skin microbiota model

Within these microorganisms, bacteria, even though they represent only 0.1% of the total, are considered as the most important microorganisms of the skin ecosystem. HCS Pharma developped a 2D in vitro skin microbiota model in 96-well plate made up of primary differentiated human keratinocytes and cutaneous bacteria such as S. epidermidis and S. aureus.

This model aims to study how your actives may influence these bacteria, but also to reproduce as much as possible the physiological cutaneous microenvironment in order to collect in vitro data predictive of those you may obtain in vivo. In this context, bacteria can be mixed for a better representation of skin microbiota.

The majority of skin bacteria represent commensal microorganisms, which take advantage of the host for nutrients and environment, and which provide in return a protection against infection, inflammation, and oxidative stress. In this context, different studies can be carried out on this skin microbiota model, including assessment of antioxidant properties of ingredients through ROS measurement.

In vitro ROS assessment on skin microbiota model