The intestinal epithelium represents an essential functional barrier for the organism. Characterized by a cell monolayer, it is mainly composed of polarized absorbing cells, the enterocytes. The intestinal epithelium is a dynamic interface between the external environment and the intestinal tissues. This permeable barrier plays a major role in the regulation of solute and fluid exchange, with direct consequences on nutrient absorption and transport. Alterations of the barrier function can cause an intestinal inflammation, which participates in digestive disorders appearance. HCS Pharma has developed tests to assess actives efficacy against it.
NFκB translocation : does my active protect Caco-2 intestinal epithelial cells against inflammation ?
Nuclear Factor kappa B (NFκB) is a protein transcription factor that orchestrates inflammation by its translocation from the cytoplasm to the cellular nucleus, allowing the expression of genes encoding inflammation proteins. This phenomenon could be assessed by immunostaining of NFκB. The transcription factor acts in intestinal epithelial cells to control epithelial integrity and the interaction between the mucosal immune system and the gut microbiota.