It’s always funny to see that the “old” maximum intensity projection is so used in picture reconstruction. Even if we all know artefacts created by this method, we often prefer a parameter free tool … In this article, a new way to do the 3D to 2D reduction is presented, the smooth manifold extraction, and it is compared to others one.
Three-dimensional fluorescence microscopy followed by image processing is routinely used to study biological objects at various scales such as cells and tissue. However, maximum intensity projection, the most broadly used rendering tool, extracts a discontinuous layer of voxels, obliviously creating important artifacts and possibly misleading interpretation. Here we propose smooth manifold extraction, an algorithm that produces a continuous focused 2D extraction from a 3D volume, hence preserving local spat