The skin performs important function, including sun protection. Melanin distribution in the epidermis plays a role in photoprotection. Solar radiations induced epidermal DNA damages, mostly in the basal layer, and is an established caused of keratinocytes cancers. Melanin distribution in human epidermis is essentialy located in the basal layer. However, Read more…
P. acnes inhibits human melanocytes survival after UVB radiation which could prevent melanoma-genesis causes by DNA damage
Melanoma is a type of skin cancer that develops from cells called melanocytes, is one of the fastest-growing and deadliest cancer in the world. It usually caused by DNA damage resulting from exposure to ultraviolet (UV) light from the sun. P. acnes is a ubiquitous gram-positive anaerobic bacterium present in Read more…
Poster – Use of Automated High Content Analysis or Imaging Applied To Assessment Of Primary DNA Damage With γH2AX and Comet Assays on different Cell types
Genotoxicity potential assessment of pharmaceuticals is mandatory for registration. In addition to the standard in vitro/ in vivo test battery, other assays can be of interest because of their high throughput in the drug discovery stage but also for mechanistic understanding.
The comet assay is a sensitive, well established technique for quantifying DNA damage in eukaryotic cells.
Poster – Use of Automated High Content Analysis Applied To Assessment Of Primary DNA Damages With γH2AX and Comet Assays
Quantifying DNA damage is mandatory to assess potential adverse effects of candidate, drugs, molecules or extracts developed in dermo-cosmetic industry. Different assays can be performed to detect primary DNA damages, such as γH2AX or the single cell gel electrophoresis also known as the comet assay.