Artificial intelligence to improve 3D cell culture : a milestone for HCS pharma

As you may now, in HCS Pharma we strongly believe that the critical point of the human health evolution in next years is the in vitro 3D cell culture, especially for complex diseases like cancer [1]. It’s why we work hard on our exclusive BIOMIMESYS® technology. As experts in HCS and cell imaging, we must master all the process : 3D biological models, 3D cell culture, volumetric pictures acquisition, 3D reconstruction and segmentation of cells and ECM compounds, 3D parameters extraction and, of course, biological interpretations. As explained in our VisuAI R&D project, the 3D reconstruction and segmentation step is not simple.

BAMM technique. Credit: CNBP

Photobleaching, a great advantage in super-multiplexed fluorescence microscopy!

In a recent article, Dr Antony Orth from the Australian Research Council’s, Centre of Excellence for Nanoscale BioPhotonics (CNBP)  explains how to use the photobleaching phenomenon to increase the potential of fluorescence microscopy. The BAMM technique (bleaching-assisted multichannel microscopy) takes advantage of the rate of photobleaching to identify a fluorophore (instead of Read more…

Evaluation by quantitative image analysis of anticancer drug activity on multicellular spheroids grown in 3D matrices

Abstract Pharmacological evaluation of anticancer drugs using 3D in vitro models provides invaluable information for predicting in vivo activity. Artificial matrices are currently available that scale up and increase the power of such 3D models. The aim of the present study was to propose an efficient and robust imaging and Read more…