BIOMIMESYS® Adipose tissue

Regulation of lipid accumulation : application with BIOMIMESYS® Adipose tissue

Retinoic acid and Caffeine are reference molecules known for their inhibitory activity on lipogenesis. Retinoic acid, a derivative of vitamin A, inhibits adipogenesis by blocking adipogenic factors such as PPARγ2 and C / EBPα, via the Retinoic acid receptor (RAR) ligand (1). Caffeine, a non-selective adenosine antagonist receptor, is used Read more…

BIOMIMESYS® Adipose tissue

Increased expression of differentiation genes with BIOMIMESYS® Adipose tissue

The morphological changes in cells during differentiation are accompanied by a profound change at the molecular level. In vivo, the adipocyte differentiation process includes early and late events due to differential gene regulation. The appearance of these markers is a sign of differentiation of pre-adipocytes and hence the development of Read more…

BIOMIMESYS® Adipose tissue

Higher lipogenesis activity with BIOMIMESYS® Adipose tissue

Triglycerides (TGs) are an efficient form of fatty acids to accumulate as energy reservoirs or as building blocks for membrane lipid synthesis. In adipose tissue, TGs are continuously synthesized during lipogenesis. In order to measure the lipogenesis activity of 3T3-L1 and mature human adipocytes, Adipored™ staining and Perilipin immunocytochemistry were Read more…

Simplifying 3d cell culture generation for high content screening with BIOMIMESYS® and the Viaflo 96-384 (with Integra Biosciences)

Growing interest in phenotypic screening, together with evidence that the drug response of cells grown in three-dimensional (3D) structures more closely resembles in vivo activity, has made high throughput, 3D fluorescence imaging an attractive screening option for drug discovery. However, creating 3D spheroids compatible with high content screening can be Read more…