It’s always funny to see that the “old” maximum intensity projection is so used in picture reconstruction. Even if we all know artefacts created by this method, we often prefer a parameter free tool … In this article, a new way to do the 3D to 2D reduction is presented, the smooth manifold extraction, and it is compared to others one.

Three-dimensional fluorescence microscopy followed by image processing is routinely used to study biological objects at various scales such as cells and tissue. However, maximum intensity projection, the most broadly used rendering tool, extracts a discontinuous layer of voxels, obliviously creating important artifacts and possibly misleading interpretation. Here we propose smooth manifold extraction, an algorithm that produces a continuous focused 2D extraction from a 3D volume, hence preserving local spat

Source: Smooth 2D manifold extraction from 3D image stack | Nature Communications

From 28 March to 1st April, the conference on DYRK1A and related kinases and human disease was held in Saint Malo. We have really enjoyed our venue as a sponsor of this conference. Thanks to the organizers, specially to Laurent Meijer and Pauline Demaison, we had a chance to present our services to the scientific community working on DYRKs protein. During 3 days, we have seen a lot of high quality scientific presentations from chemistry to the development of treatments for DYRKs related disease. We hope all these talks from international researchers will lead to scientific improvements in DIRK related disease.

The development of cellular models, especially in neurotoxicity, is an approach used to accelerate the development of new therapeutics for DYRK related disease. With our progress in 3D models, HCS Pharma hopes to provide new tools for researchers to better understand DYRKs protein,  and perhaps participate as a speakers at the next DYRK conference !

To see our assay in neurotoxicity just click here : http://hcs-pharma.com/services/toxicology/neurotox/

3D cell models are now attracting a huge interest from scientists working both on toxicity and pharmacology assay development. They are considered more relevant at mimicking the in vivo situation. However, phenotypic assays on these models can be challenging, and are at least more complex.

A team from Molecular Devices and Cellular Dynamics has worked on the “phenotypic characterization of toxic compound effects on liver spheroids derived from iPSC using confocal imaging an three-dimensional image analyis”. The results have been published September 2016 in Assay and Drug Development Technologies, and describe how the ImageXpress Micro Confocal High-Content Imaging System and MetaXpress High-Content Image and Analysis Software (Molecular Devices) were used to manage the phenotypic characterization.

Assuming that the approach may be extensible to more complex 3D systems, such as cultures containing multiple cell types (e.g., Kuppfer cells, fibroblasts, endothelial cells), they conclude 3D analysis would allow characterization of different cell populations and their roles in toxicity and liver injury.

Source : Phenotypic Characterization of Toxic Compound Effects on Liver Spheroids Derived from iPSC Using Confocal Imaging and Three-Dimensional Image Analysis

It’s very interesting to show that, at HCS Pharma, we work on many of the most exciting medical technologies identified by Dr. Bertalan Mesko on his blog, medicalfuturist.com. Of course, precision medicine is the most important for us, because we see here extraordinary tools to treat cancer and neurodegenerative diseases (“instead of canons, we start using sniper rifles.”)

But two other fields seem really promising. The 3D bioprinting for exemple will give us the possibility to create relevant in vitro models of living tissues. We also work to integrate IA deep learning in our HCS processes. It will be a major method to find phenotypic actions of futur drugs.

2016 was a rich year for medical technology. Virtual Reality. Augmented Reality. Smart algorithms analysing wearable data. Amazing technologies arrived in our lives and on the market almost every day. And it will not stop in the coming year.

Source: The Most Exciting Medical Technologies of 2017 – The Medical Futurist

ESTIV conference, named In Vitro Toxicology for Human Safety Assessment, takes place this week in Juan-les-Pins. This congress brings together a lot of toxicologists, from public and private, to discuss the last development of predictive in vitro models in safety testing, as 3D models, organ-on-a-chip, bioprint organs.

To learn more and to exchange on these topics, Julian, our COO, is present to this conference. Have a nice time to discuss with him on these last development of in vitro safety testing models ! If you want to be contacted, please leave your mobile number here :

We just begin a test period of the Operetta “High Content Imaging System” from Perkin Elmer. First impressions are very good, especially using confocal option for 3D imaging. As you can see below, neurites growth is quite easy to observe around and inside a spheroid of SH-SY5Y cells. To properly count it is more complicated for the moment but we progress in the mastering of “Harmony” and “Columbus” softwares, as for “Acapella” scripts. Our next challenge is to build comprehensive 3D visualisation with Volocity software.

More and more articles show the advantages of culture compared to 2D in field. This article describe how to do high throughput using 3D culture by rapid size profiling analysis over time on tumor spheroids.

“Tumor size is the most frequently used in vivo endpoint when assessing antitumor efficacy in animal xenograft models, whereas proliferation is the more typically evaluated growth endpoint in vitro using two-dimensional (2D) monolayer cultures. Such 2D in vitro assays frequently fail to correlate with in vivo observations, owing to the inability of 2D cultures to recapitulate the native tumor microenvironment described above. Three-dimensional (3D) tumor microtissues, or multicellular tumor spheroids, are considered a more representative, organotypic model for assessment of tumor growth. They contain layers of cells that exhibit more in vivo-like size- and gradient-dependent proliferation and viability profiles.”

To know more about this article, follow this link: http://www.genengnews.com/gen-articles/phenotypic-drug-discovery-in-3d/5303/

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