For ethical and cost-related reasons, use of animals for the assessment of mode of action, metabolism and/or toxicity of new drug candidates has been increasingly scrutinized in research and industrial applications. Physiologically based pharmacokinetic (PBPK2) modeling is the most potent in silico tool used for extrapolation of pharmacokinetic parameters to Read more…
How to see cells moves ?
I was really happy to be invited in SIBS 2017 in Zurich last week to present our work on 3D cell culture in phenotypic screening and our use of disruptive technologies in our process (augmented reality and virtual reality). I presented our collaborative work with Dr Karim Si-Tayeb, researcher from Read more…
More than 300,000 images available !
As shown by AstraZeneca in nature reviews*, one third of safety failures along the drug discovery process is linked to CNS toxicity uncovered in clinical trials. To avoid this attrition, the potential neurotoxicity of any drug going through the blood brain barrier (BBB) needs to be assessed in the very early stages Read more…
3D cell models are now attracting a huge interest from scientists working both on toxicity and pharmacology assay development. They are considered more relevant at mimicking the in vivo situation. However, phenotypic assays on these models can be challenging, and are at least more complex.
Despite continuous improvement of DNA FISH, a method that has been extensively used for years, it still requires harsh treatments to allow probe hybridization.
Cell biology is part of the R&D activity in dermocosmetology. At HCS Pharma, we are developing dermocosmetology assay using our automation platform and high content analysis software.
Poster – Development of double stand break assessment assay with HCS by using the HepG2 cell line and evaluation with primary skin cell (NHEK)
The creation of a double strand break (DSB) is accompanied by the phosphorylation of histone H2AX. The measurement of serine 139 phosphorylated histone H2AX (γH2AX) is reported to be a marker of interest to identify potential genotoxic activity.
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Thomas Kirchhausen, Eric Betzig et al. publish an article in Sciences about SIM (structured illumination microscopy) and the observation of moving molecules inside cells. The incredible spatial and time resolution give the opportunity to see for exemple proteins which pass the cell membrane.
Aviv Regev at the Broad Institute, in Cambridge, use fluidic systems to separate cells and submits them to detailed genetic analysis, at the rate of thousands per day. The goal is to build a comprehensive cellular atlas of the human, based on gene expression profile.
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Researchers from Yale university presented a new technology to increase presicion of high speed (and wide field) imagery. Using both LEDs and laser light, they minimize spacial coherence and granularity. It’s a promising way to optimize cells imaging.
Here is a nice example of cell imaging uses for stem cells characterisation. Different culture conditions are performed on adult stem cells and cell adhesion, proliferation, survival, and cell migration are followed by cell imaging.