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Drug discovery : Need of reliable IVIVE for pharmacodynamics and pharmacokinetics assessment
For ethical and cost-related reasons, use of animals for the assessment of mode of action, metabolism and/or toxicity of new drug candidates has been increasingly scrutinized in research and industrial applications. Physiologically based pharmacokinetic (PBPK2) modeling is the most potent in silico tool used for extrapolation of pharmacokinetic parameters to Read more…
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Internal forces directing cell migration are revealed by live-cell microscopy
How to see cells moves ?
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Feedback of SIBS 2017 in Zurich last week
I was really happy to be invited in SIBS 2017 in Zurich last week to present our work on 3D cell culture in phenotypic screening and our use of disruptive technologies in our process (augmented reality and virtual reality). I presented our collaborative work with Dr Karim Si-Tayeb, researcher from Read more…
HCS, HCA & Biology
Cell Atlas, a comprehensive image-based map of the subcellular protein distribution
More than 300,000 images available !
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Poster – Neurotoxicity assay on 2D and 3D culture using High Content Screening (HCS) technology
As shown by AstraZeneca in nature reviews*, one third of safety failures along the drug discovery process is linked to CNS toxicity uncovered in clinical trials. To avoid this attrition, the potential neurotoxicity of any drug going through the blood brain barrier (BBB) needs to be assessed in the very early stages Read more…
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Phenotypic characterization on liver spheroïds
3D cell models are now attracting a huge interest from scientists working both on toxicity and pharmacology assay development. They are considered more relevant at mimicking the in vivo situation. However, phenotypic assays on these models can be challenging, and are at least more complex.
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New promising method for in situ labeling of genomic loci in fixed cells
Despite continuous improvement of DNA FISH, a method that has been extensively used for years, it still requires harsh treatments to allow probe hybridization.
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Welcome Kathleen, cell culture leader !
We are very please to welcome Kathleen in HCS Pharma.
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Wound healing assay development in dermocosmetology
Cell biology is part of the R&D activity in dermocosmetology. At HCS Pharma, we are developing dermocosmetology assay using our automation platform and high content analysis software.
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Poster – Development of double stand break assessment assay with HCS by using the HepG2 cell line and evaluation with primary skin cell (NHEK)
The creation of a double strand break (DSB) is accompanied by the phosphorylation of histone H2AX. The measurement of serine 139 phosphorylated histone H2AX (γH2AX) is reported to be a marker of interest to identify potential genotoxic activity.
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To look inside the cell with SIM (structured illumination microscopy)
Thomas Kirchhausen, Eric Betzig et al. publish an article in Sciences about SIM (structured illumination microscopy) and the observation of moving molecules inside cells. The incredible spatial and time resolution give the opportunity to see for exemple proteins which pass the cell membrane.
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Single-Cell Genomics Allows Identification of New Cell Types
Aviv Regev at the Broad Institute, in Cambridge, use fluidic systems to separate cells and submits them to detailed genetic analysis, at the rate of thousands per day. The goal is to build a comprehensive cellular atlas of the human, based on gene expression profile.
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Laser and LED combined for better medical images
Researchers from Yale university presented a new technology to increase presicion of high speed (and wide field) imagery. Using both LEDs and laser light, they minimize spacial coherence and granularity. It’s a promising way to optimize cells imaging.
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High Throughput Characterization of Stem Cells by cell imaging
Here is a nice example of cell imaging uses for stem cells characterisation. Different culture conditions are performed on adult stem cells and cell adhesion, proliferation, survival, and cell migration are followed by cell imaging.